Graphical User Interface for Single-Pixel Spectroscopy
Mary M.Yang, Michael R. Dilworth, and Douglas C. Youvan
KAIROS Scientific Inc.
3350 Scott Blvd.
Santa Clara, CA 95054 USA
Digital imaging spectroscopy (DIS) combines image processing and optical spectroscopy such that each picture element (pixel) or group of pixels in a two dimensional scene also includes additional dimension(s) of spectral and/or temporal information. Imaging spectrophotometers developed at KAIROS are literally equivalent to hundreds of thousands of conventional spectrophotometers running in parallel, i.e., each pixel is functionally equivalent to a single 'instrument'. Because of the amount of data acquired by this massively parallel technique - often in the gigabyte range for a single experiment - it was necessary to develop a new generation of graphical tools for data analysis and display. A unifying theme in our software development is the use of multispectral color contour plots to concisely represent data from a variety of different imaging experiments. Typically, the rows of a color contour plot represent encoded information for individual pixels. The columns of a contour plot represent the values of the variable that are being measured: absorbance, reflectance, fluorescence, FRET, or time course (kinetic) data. Contour plots are very powerful for classifying pixels with similar properties; therefore, these areas within the image can be 'back-painted' with a specific pseudocolor. Numerous sorting algorithms can be applied to the contour plot to map spectral and kinetic information back onto the spatial representation in run-time. Guided by the heuristics of parameters input by the user, the application of sorting algorithms to contour plots results in a near optimal interface between machine and human intelligence for the visualization of hyperspectral and/or hypertemporal images. In addition to a general introduction to the DIS graphical user interface, we demonstrate this new technology on H&E stained tissue.
Full PDF Text (for better printing)
Full HTML (for better viewing)
© Biotechnology et alia, 2000
*Current address: 10225 Barnes Canyon Rd., A110 San Diego, CA 92121 USA